In-vitro cytotoxic activity of β -Sitosterol triacontenate isolated from Capparis decidua (Forsk.) Edgew

Objective: To study the isolation and characterization of the constituent responsible for the cytotoxic activity of the ethanolic extract of stem of Copparis decidua (C. decidua). Methods: The preliminary cytotoxic effect of isolated compound (beta-Sitosterol triacontenate) was investigated by MTT assay on A549 solid tumor cells. Results: IC50 value of the beta-Sitosterol triacontenate was found to he 1 mu M. The cytotoxic activity increased in a dose dependent Milliner in case of beta-Sitosterol triacontenate. Conclusions: The data therefore provide direct evidence for the role of beta-Sitosterol triacontenate as a potent antimetastalic agent, which can markedly inhibit the metastatic anal invasive capacity of malignant cells.