Reliability of the E-test method for detection of colistin resistance in clinical isolates of Acinetobacter baumannii

被引：92

作者：

Arroyo, LA

García-Curiel, A

Pachón-Ibañez, ME

Llanos, AC

Ruiz, M

Pachón, J

Aznar, J

机构：

[1] Hosp Univ Virgen Rocio, Clin Mircobiol Serv, Seville 41013, Spain

[2] Hosp Univ Virgen Rocio, Infect Dis Serv, Seville 41013, Spain

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2005年 / 43卷 / 02期

关键词：

D O I：

10.1128/JCM.43.2.903-905.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We compared the E-test to the broth microdilution method for testing the susceptibility of 115 clinical isolates of Acinetobacter baumannii to colistin. Twenty-two (19.1%) strains were resistant to colistin and 93 (80.8%) strains were susceptible according to the reference broth microdilution method. A categorical agreement of 98.2% was found, with only two (1.7%) very major errors. Agreement within I twofold dilution between the E-test and the broth microdilution was 16.5%. Complete agreement was found for the strains for which MICs fell within the range of 0.25 to 1 mug of colistin/ml. However, there was poor concordance, particularly in extreme dilutions with higher MICs by the E-test method.

引用

收藏

页码：903 / 905

页数：3

相关论文

共 18 条

[1]

[Anonymous], [No title captured]

[2] In vitro activities of nontraditional antimicrobials against multiresistant Acinetobacter baumannii strains isolated in an intensive care unit outbreak [J].

Appleman, MD ;

Belzberg, H ;

Citron, DM ;

Heseltine, PNR ;

Yellin, AE ;

Murray, J ;

Berne, TV .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2000, 44 (04) :1035-1040

[3]

ARROYO L, 2003, 13 EUR C CLIN MICR I, P168

[4] Acinetobacter spp, as nosocomial pathogens: Microbiological, clinical, and epidemiological features [J].

BergogneBerezin, E ;

Towner, KJ .

CLINICAL MICROBIOLOGY REVIEWS, 1996, 9 (02) :148-+

[5] A reassessment of the in-vitro activity of colistin sulphomethate sodium [J].

Catchpole, CR ;

Andrews, JM ;

Brenwald, N ;

Wise, R .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1997, 39 (02) :255-260

[6] Polymyxin B sulfate and colistin: Old antibiotics for emerging multiresistant gram-negative bacteria [J].

Evans, ME ;

Feola, DJ ;

Rapp, RP .

ANNALS OF PHARMACOTHERAPY, 1999, 33 (09) :960-967

[7] Successful treatment of ventriculitis due to carbapenem-resistant Acinetobacter baumannii with intraventricular colistin sulfomethate sodium [J].

Fernandez-Viladrich, P ;

Corbella, X ;

Corral, L ;

Tuba, F ;

Mateu, A .

CLINICAL INFECTIOUS DISEASES, 1999, 28 (04) :916-917

[8] Antimicrobial susceptibility and frequency of occurrence of clinical blood isolates in Europe from the SENTRY Antimicrobial Surveillance Program, 1997 and 1998 [J].

Fluit, AC ;

Jones, ME ;

Schmitz, FJ ;

Acar, J ;

Gupta, R ;

Verhoef, J .

CLINICAL INFECTIOUS DISEASES, 2000, 30 (03) :454-460

[9] Contemporary assessment of antimicrobial susceptibility testing methods for polymyxin B and colistin: Review of available interpretative criteria and quality control guidelines [J].

Gales, AC ;

Reis, AO ;

Jones, RN .

JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (01) :183-190

[10] Treatment of multidrug-resistant Acinetobacter baumannii ventilator-associated pneumonia (VAP) with intravenous colistin:: A comparison with imipenem-susceptible VAP [J].

Garnacho-Montero, J ;

Ortiz-Leyba, C ;

Jiménez-Jiménez, FJ ;

Barrero-Alodóvar, AE ;

García-Garmendia, JL ;

Bernabeu-Witteli, M ;

Gallego-Lara, SL ;

Madrazo-Osuna, J .

CLINICAL INFECTIOUS DISEASES, 2003, 36 (09) :1111-1118