Application of a newly developed ARB software-integrated tool for in silico terminal restriction fragment length polymorphism analysis reveals the dominance of a novel pmoA cluster in a forest soil

被引:64
作者
Ricke, P [1 ]
Kolb, S [1 ]
Braker, G [1 ]
机构
[1] Max Planck Inst Terr Microbiol, D-35043 Marburg, Germany
关键词
D O I
10.1128/AEM.71.3.1671-1673.2005
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
TRF-CUT, an ARB-implemented tool, was developed to predict in silico the terminal restriction fragments of aligned small-subunit rRNA gene or functional gene sequences. Application of this new tool to perform directed terminal restriction fragment length polymorphism analysis of pmoA products obtained from a forest soil revealed that novel cluster I methanotrophic bacteria were dominant.
引用
收藏
页码:1671 / 1673
页数:3
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