High efficiency 5 min transformation of Escherichia coli

被引：68

作者：

Pope, B

Kent, HM

机构：

[1] MRC Laboratory of Molecular Biology, Cambridge CB2 2QH, Hills Road

来源：

NUCLEIC ACIDS RESEARCH | 1996年 / 24卷 / 03期

关键词：

D O I：

10.1093/nar/24.3.536

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

[No abstract available]

引用

页码：536 / 537

页数：2

共 8 条

[1] ONE-STEP PREPARATION OF COMPETENT ESCHERICHIA-COLI - TRANSFORMATION AND STORAGE OF BACTERIAL-CELLS IN THE SAME SOLUTION [J].

CHUNG, CT ;

NIEMELA, SL ;

MILLER, RH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (07) :2172-2175

[2] NONCHROMOSOMAL ANTIBIOTIC RESISTANCE IN BACTERIA - GENETIC TRANSFORMATION OF ESCHERICHIA-COLI BY R-FACTOR DNA [J].

COHEN, SN ;

CHANG, ACY ;

HSU, L .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1972, 69 (08) :2110-&

[3] PROLONGED INCUBATION IN CALCIUM-CHLORIDE IMPROVES THE COMPETENCE OF ESCHERICHIA-COLI-CELLS [J].

DAGERT, M ;

EHRLICH, SD .

GENE, 1979, 6 (01) :23-28

[4] SITE-DIRECTED MUTAGENESIS OF VIRTUALLY ANY PLASMID BY ELIMINATING A UNIQUE SITE [J].

DENG, WP ;

NICKOLOFF, JA .

ANALYTICAL BIOCHEMISTRY, 1992, 200 (01) :81-88

[5] STUDIES ON TRANSFORMATION OF ESCHERICHIA-COLI WITH PLASMIDS [J].

HANAHAN, D .

JOURNAL OF MOLECULAR BIOLOGY, 1983, 166 (04) :557-580

[6] CALCIUM-DEPENDENT BACTERIOPHAGE DNA INFECTION [J].

MANDEL, M ;

HIGA, A .

JOURNAL OF MOLECULAR BIOLOGY, 1970, 53 (01) :159-&

[7]

Maniatis T., 1982, MOL CLONING

[8] IDENTIFICATION OF A REGION IN SEGMENT-1 OF GELSOLIN CRITICAL FOR ACTIN BINDING [J].

WAY, M ;

POPE, B ;

GOOCH, J ;

HAWKINS, M ;

WEEDS, AG .

EMBO JOURNAL, 1990, 9 (12) :4103-4109

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