Guidelines in implementing enantioselective indicator-displacement assays for α-hydroxycarboxylates and diols

被引：179

作者：

Zhu, L ^{[1
]}

Zhong, ZL ^{[1
]}

Anslyn, EV ^{[1
]}

机构：

[1] Univ Texas, Dept Chem & Biochem, Austin, TX 78712 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2005年 / 127卷 / 12期

关键词：

D O I：

10.1021/ja0435945

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Enantioselective association events between boronic acid receptors and bifunctional substrates such as alpha-hydroxycarboxylates and vicinal diols were exploited to develop enantioselective indicator-displacement assays. It was found that the sensitivity of the assay could be tuned by using either colorimetric or fluorescent indicators. Mathematical principles were established for concurrent determination of concentration and enantiomeric excess (ee) of a chiral analyte, which has been a challenge to the high-throughput analysis of chiral samples. Furthermore, experimental protocols were optimized to maximize the chiral resolution of the assay and to improve the accuracy of the ee measurements.

引用

页码：4260 / 4269

页数：10

共 69 条

[1]

Acton FS., 1970, NUMERICAL METHODS WO

[2] Achieving large color changes in response to the presence of amino acids:: A molecular sensing ensemble with selectivity for aspartate [J].