MicroRNA miR-886-5p inhibits apoptosis by down-regulating Bax expression in human cervical carcinoma cells

被引：83

作者：

Li, Jing-Hua ^{[2
]}

Xiao, Xue ^{[3
]}

Zhang, Yao-Nan ^{[2
]}

Wang, Ya-Mei ^{[2
]}

Feng, Li-Min ^{[4
]}

Wu, Yu-Mei ^{[1
]}

Zhang, Yu-Xiang ^{[2
]}

机构：

[1] Capital Med Univ, Beijing Gynecol & Obstet Hosp, Dept Gynecol Oncol, Beijing 100026, Peoples R China

[2] Capital Med Univ, Inst Canc, Dept Biochem & Mol Biol, Beijing 100026, Peoples R China

[3] Capital Med Univ, Dept Biomed Engn, Beijing 100026, Peoples R China

[4] Capital Med Univ, Beijing Tian Tan Hosp, Dept Gynecol, Beijing 100026, Peoples R China

来源：

GYNECOLOGIC ONCOLOGY | 2011年 / 120卷 / 01期

关键词：

Cervical cancer; Microarray; MiR-886-5p; Apoptosis; Bax; HUMAN-PAPILLOMAVIRUS; SIGNATURE; INFECTION; PROFILES; ONCOMIRS; TYPE-16; PCR;

D O I：

10.1016/j.ygyno.2010.09.009

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

Objective. MicroRNA (miRNA) plays an essential role in the progression of a variety of cancers, but its role in cervical cancer progression is not well defined. We aimed to test whether special miRNAs and their target mRNAs contribute to cervical cancer progression. Methods. The expression profiles of 1145 microRNAs in cervical squamous cell carcinomas (CSCC) and adjacent non-tumor tissues were investigated using an Illumina microRNA microarray platform. Differentially expressed miRNAs were validated by RT-PCR. Downstream target validation was performed for miR-886-5p. Results. We found that the expression levels of seven miRNAs differed significantly between CSCC tissues and adjacent non-tumor tissues. Forced expression of one miRNA, miR-886-5p, over-expressed in CSCC tissues lowered expression of the pro-apoptotic protein Bax, reduced apoptosis and promoted cell proliferation in H8, an HPV16-immortalized human cervical squamous epithelial cell line. Knockdown of miR-886-5p increased Bax protein and apoptotic cell death in cells of the cervical squamous carcinoma cell line, SiHa. Conclusion. MicroRNA miR-886-5p inhibits apoptosis of cervical cancer cells by down-regulating the production of Bax. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：145 / 151

页数：7

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