TRPM8 protein localization in trigeminal ganglion and taste papillae

被引：125

作者：

Abe, J ^{[1
]}

Hosokawa, H ^{[1
]}

Okazawa, M ^{[1
]}

Kandachi, M ^{[1
]}

Sawada, Y ^{[1
]}

Yamanaka, K ^{[1
]}

Matsumura, K ^{[1
]}

Kobayashi, S ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Informat, Dept Intelligence Sci & Technol, Div Biol Informat,Sakyo Ku, Kyoto 6068501, Japan

来源：

MOLECULAR BRAIN RESEARCH | 2005年 / 136卷 / 1-2期

基金：

日本学术振兴会;

关键词：

cold and menthol receptor-1; trigeminal ganglion; fungiform papilla; lingual nerve;

D O I：

10.1016/j.molbrainres.2005.01.013

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

TRPM8 is a TRP family cation channel which can be activated by cold stimuli or L-menthol. However, TRPM8 protein localization of nerve terminals in sensory organs remains unknown. Here we generated an antibody against TRPM8 and analyzed TRPM8 protein localization in trigeminal ganglia (TG) and in sensory nerve fibers in the tongue. TRPM8 immunoreactivity was detected in a subset of neurons with a small diameter in TG and in nerve fibers in the tongue. TRPM8-immunoreactive nerve fibers were rich in fungiform papillae, but sparse in foliate and circumvallate papillae. The TRPM8-immunoreactive nerve fibers reached the outer epithelial layer in each papilla, while no TRPM8-immunoreactive nerve fibers penetrated into taste buds. Double labeling analysis revealed that TRPM8 immunoreactivity was co-expressed with a part of TRPV1 or CGRP-immunoreactive neurons in TG. However, TRPM8 immunoreactivity was not observed in TRPV1- or CGRP-positive nerve fibers in fungiform, foliate, and circumvallate papillae. These results suggest that TRPM8 protein is present in sensory lingual nerve fibers mainly projected from TG and might work as cold and L-menthol receptors on tongue. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：91 / 98

页数：8

共 26 条

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