Control of leaf morphogenesis by microRNAs

被引：1400

作者：

Palatnik, JF

Allen, E

Wu, XL

Schommer, C

Schwab, R

Carrington, JC

Weigel, D ^{[1
]}

机构：

[1] Max Planck Inst Dev Biol, Dept Mol Biol, D-72076 Tubingen, Germany

[2] Salk Inst Biol Studies, Plant Biol Lab, La Jolla, CA 92037 USA

[3] Oregon State Univ, Ctr Gene Res & Biotechnol, Corvallis, OR 97331 USA

[4] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA

来源：

NATURE | 2003年 / 425卷 / 6955期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

D O I：

10.1038/nature01958

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Plants with altered microRNA metabolism have pleiotropic developmental defects, but direct evidence for microRNAs regulating specific aspects of plant morphogenesis has been lacking. In a genetic screen, we identified the JAW locus, which produces a microRNA that can guide messenger RNA cleavage of several TCP genes controlling leaf development. MicroRNA-guided cleavage of TCP4 mRNA is necessary to prevent aberrant activity of the TCP4 gene expressed from its native promoter. In addition, overexpression of wild-type and microRNA-resistant TCP variants demonstrates that mRNA cleavage is largely sufficient to restrict TCP function to its normal domain of activity. TCP genes with microRNA target sequences are found in a wide range of species, indicating that microRNA-mediated control of leaf morphogenesis is conserved in plants with very different leaf forms.

引用

页码：257 / 263

页数：7

共 40 条

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