A quartz crystal resonant sensor (QCRS) study of HSA-drug interactions

被引：23

作者：

Pavey, KD

Lyle, EL

Olliff, CJ

Paul, F

机构：

[1] Univ Brighton, Sch Pharm & Biomol Sci, Brighton BN2 4GJ, E Sussex, England

[2] Univ Toronto, Dept Chem, Toronto, ON M5S 3H6, Canada

[3] SmithKline Beecham Pharmaceut, NFSP N, CASS, Novel Methods Grp, Harlow CM19 5AW, Essex, England

来源：

ANALYST | 2001年 / 126卷 / 04期

关键词：

D O I：

10.1039/b009986o

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Human serum albumin (HSA) was immobilised on the gold surface of a quartz crystal resonance sensor (QCRS) and exposed to warfarin and diazepam, Distinct decreases in frequency of differing magnitudes were observed upon exposure of the protein to each of the compounds suggesting strongly that a ligand interaction was occurring. Moreover, as sequential exposure in any order was observed to yield distinct repeatable frequency decreases for the ligands indicated, screening for site specific binding may be possible, Identically immobilised bovine serum albumin (BSA) gave no response to either compound.

引用

页码：426 / 428

页数：3

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