Evaluation of a reversed passive latex agglutination test for the detection of Verocytotoxin (VT) expressed by strains of VT-producing Escherichia coli

被引：14

作者：

Chart, H ^{[1
]}

Willshaw, GA ^{[1
]}

Cheasty, T ^{[1
]}

机构：

[1] Cent Publ Hlth Lab, Div Gastrointestinal Infect, Lab Enter Pathogens, London NW9 5HT, England

来源：

LETTERS IN APPLIED MICROBIOLOGY | 2001年 / 32卷 / 06期

关键词：

D O I：

10.1046/j.1472-765X.2001.00921.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aims: To compare an experimental Reversed-Passive Latex Agglutination (RPLA) with Vero cells for the detection of Verocytotoxin expressed by VT-producing strains of Escherichia coli (VTEC). Methods and Results: The RPLA was used alongside a Vero cell tissue culture assay for the detection of VT in bacterial culture supernatant fluids and patients' faecal extracts. Conclusions: The RPLA was comparable with the Vero cell assay, although slightly less sensitive. Significance and Impact of the Study: The RPLA test proved to be a simple, rapid and convenient method of detecting VT in bacterial culture supernatant fluids and in the faeces of patients infected with VTEC.

引用

页码：370 / 374

页数：5

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