Identification of aluminium-regulated genes by cDNA-AFLP in rice (Oryza sativa L.):: aluminium-regulated genes for the metabolism of cell wall components

被引：64

作者：

Mao, CZ ^{[1
]}

Yi, K ^{[1
]}

Yang, L ^{[1
]}

Zheng, BS ^{[1
]}

Wu, YR ^{[1
]}

Liu, FY ^{[1
]}

Wu, P ^{[1
]}

机构：

[1] Zhejiang Univ, Coll Life Sci, State Key Lab Plant Physiol & Biochem, Hangzhou 310029, Peoples R China

来源：

JOURNAL OF EXPERIMENTAL BOTANY | 2004年 / 55卷 / 394期

基金：

中国国家自然科学基金;

关键词：

aluminium stress; cDNA-AFLP; cell wall components; Oryza sativa L;

D O I：

10.1093/jxb/erh030

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Aluminium (Al) toxicity is the major factor limiting crop productivity in acid soils. To investigate the molecular mechanisms of Al toxicity and Al tolerance of rice, cDNA-amplified fragment length polymorphism (cDNA-AFLP) was used for identifying Al-regulated genes in roots of an Al-tolerant tropical upland rice, Azucena, and an Al-sensitive lowland rice, IR1552. Nineteen function-known genes were found among 34 transcript-derived fragments (TDFs) regulated by Al stress. The results indicate that Al stress could induce the biosynthesis of lignin and other cell wall components in roots. Temporal expression patterns of 14 genes were identified between the two varieties. In silico mapping was performed for all the 33 unique genes. Two genes for a function-unknown protein and for a ubiquitin-like protein, respectively, were mapped on the interval with the common QTL (quantitative trait loci) for Al tolerance in rice on chromosome 1.

引用

页码：137 / 143

页数：7

共 35 条

[1] Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP: Analysis of gene expression during potato tuber development [J].