(R)-citramalate synthase in methanogenic archaea

被引：81

作者：

Howell, DM ^{[1
]}

Xu, HM ^{[1
]}

White, RH ^{[1
]}

机构：

[1] Virginia Polytech Inst & State Univ, Coll Agr & Life Sci, Dept Biochem, Blacksburg, VA 24061 USA

来源：

JOURNAL OF BACTERIOLOGY | 1999年 / 181卷 / 01期

关键词：

D O I：

10.1128/JB.181.1.331-333.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Methanococcus jannaschii gene MJ1392 was cloned, and its protein product was hyperexpressed in Escherichia coli. The resulting protein was purified and shown to catalyze the condensation of pyruvate and acetyl coenzyme A, with the formation of (R)-citramalate. Thus, this gene (cimA) encodes an (R)-citramalate synthase (CimA). This is the first identification of this enzyme, which is likely involved in the biosynthesis of isoleucine.

引用

页码：331 / 333

页数：3

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