Detection of human immunodeficiency virus type 1 after infection of unstimulated peripheral blood mononuclear cells

被引:4
作者
Shapiro, SZ [1 ]
Maudru, T [1 ]
Peden, KWC [1 ]
机构
[1] US FDA, Ctr Biol Evaluat & Res, Lab Retrovirus Res, Bethesda, MD 20892 USA
关键词
D O I
10.1099/0022-1317-80-4-857
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Application of a highly sensitive PCR-based reverse transcriptase (RT) assay to the analysis of the infection of CD4(+) cell lines with human immunodeficiency virus type 1 (HIV-1) demonstrated that virus production can be detected as early as 24 h after infection. Most of the signal at 24 h was due to virus production, as it could be substantially reduced by prior treatment with the RT inhibitor zidovudine. Virus production at 24 and 48 h was unaffected by the protease inhibitor indinavir. Infection of unstimulated peripheral blood mononuclear cells (PBMC) with a macrophage-tropic HIV-1 isolate yielded increasing virus production for 2-3 weeks, while infection with a T-cell line-tropic isolate yielded only low and sporadic virus production. Productive infection of unstimulated PBMC by the macrophage-tropic virus required functional Gag matrix and Vpr proteins; therefore, the monocyte-derived macrophage is probably the virus-producing cell in these cultures.
引用
收藏
页码:857 / 861
页数:5
相关论文
共 22 条
[1]   PRODUCTION OF ACQUIRED IMMUNODEFICIENCY SYNDROME-ASSOCIATED RETROVIRUS IN HUMAN AND NONHUMAN CELLS TRANSFECTED WITH AN INFECTIOUS MOLECULAR CLONE [J].
ADACHI, A ;
GENDELMAN, HE ;
KOENIG, S ;
FOLKS, T ;
WILLEY, R ;
RABSON, A ;
MARTIN, MA .
JOURNAL OF VIROLOGY, 1986, 59 (02) :284-291
[2]   CC CKRS: A RANTES, MIP-1 alpha, MIP-1 beta receptor as a fusion cofactor for macrophage-tropic HIV-1 [J].
Alkhatib, G ;
Combadiere, C ;
Broder, CC ;
Feng, Y ;
Kennedy, PE ;
Murphy, PM ;
Berger, EA .
SCIENCE, 1996, 272 (5270) :1955-1958
[3]   A NUCLEAR-LOCALIZATION SIGNAL WITHIN HIV-1 MATRIX PROTEIN THAT GOVERNS INFECTION OF NONDIVIDING CELLS [J].
BUKRINSKY, MI ;
HAGGERTY, S ;
DEMPSEY, MP ;
SHAROVA, N ;
ADZHUBEI, A ;
SPITZ, L ;
LEWIS, P ;
GOLDFARB, D ;
EMERMAN, M ;
STEVENSON, M .
NATURE, 1993, 365 (6447) :666-669
[4]   QUIESCENT LYMPHOCYTES-T AS AN INDUCIBLE VIRUS RESERVOIR IN HIV-1 INFECTION [J].
BUKRINSKY, MI ;
STANWICK, TL ;
DEMPSEY, MP ;
STEVENSON, M .
SCIENCE, 1991, 254 (5030) :423-427
[5]   VPR IS REQUIRED FOR EFFICIENT REPLICATION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 IN MONONUCLEAR PHAGOCYTES [J].
CONNOR, RI ;
CHEN, BK ;
CHOE, S ;
LANDAU, NR .
VIROLOGY, 1995, 206 (02) :935-944
[6]   ROLE OF THE BASIC DOMAIN OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 MATRIX IN MACROPHAGE INFECTION [J].
FREED, EO ;
ENGLUND, G ;
MARTIN, MA .
JOURNAL OF VIROLOGY, 1995, 69 (06) :3949-3954
[7]   HIV-1 INFECTION OF NONDIVIDING CELLS [J].
FREED, EO ;
MARTIN, MA .
NATURE, 1994, 369 (6476) :107-108
[8]   THE VPR PROTEIN OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 INFLUENCES NUCLEAR-LOCALIZATION OF VIRAL NUCLEIC-ACIDS IN NONDIVIDING HOST-CELLS [J].
HEINZINGER, NK ;
BUKRINSKY, MI ;
HAGGERTY, SA ;
RAGLAND, AM ;
KEWALRAMANI, V ;
LEE, MA ;
GENDELMAN, HE ;
RATNER, L ;
STEVENSON, M ;
EMERMAN, M .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (15) :7311-7315
[9]   DETECTION OF REVERSE-TRANSCRIPTASE BY A HIGHLY SENSITIVE ASSAY IN SERA FROM PERSONS INFECTED WITH HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 [J].
HENEINE, W ;
YAMAMOTO, S ;
SWITZER, WM ;
SPIRA, TJ ;
FOLKS, TM .
JOURNAL OF INFECTIOUS DISEASES, 1995, 171 (05) :1210-1216
[10]   GROWTH OF MACROPHAGE-TROPIC AND PRIMARY HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 (HIV-1) ISOLATES IN A UNIQUE CD4(+) T-CELL CLONE (PM1) - FAILURE TO DOWN-REGULATE CD4 AND TO INTERFERE WITH CELL-LINE-TROPIC HIV-1 [J].
LUSSO, P ;
COCCHI, F ;
BALOTTA, C ;
MARKHAM, PD ;
LOUIE, A ;
FARCI, P ;
PAL, R ;
GALLO, RC ;
REITZ, MS .
JOURNAL OF VIROLOGY, 1995, 69 (06) :3712-3720