A nucleosome interaction module is required for normal function of Arabidopsis thaliana BRAHMA

被引:48
作者
Farrona, S. [1 ]
Hurtado, L. [1 ]
Reyes, J. C. [1 ]
机构
[1] Ctr Andaluz Biol Mol & Med Regenerat, Inst Bioquim Vegetal & Fotosintesis, Consejo Superior Invest Cientificas Americo Vespu, E-41092 Seville, Spain
关键词
chromatin; bromodomain; swi/snf complex; AT-hook; seed development;
D O I
10.1016/j.jmb.2007.07.012
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The BRAHMA (BRM) gene encodes the SNF2-type ATPase of the putative Arabidopsis thaliana SWI/SNF chromatin remodelling complex. This family of ATPases is characterized by the presence of a conserved catalytic domain and an arrangement of auxiliary domains, whose functions in the remodelling activity remains unclear. Here, we characterize, at the molecular and functional level, the carboxy-terminal part of Arabidopsis BRM. We have found three DNA-binding regions that bind various free DNA and nucleosomal probes with different specificity. One of these regions contains an AT-hook motif. The carboxy terminus also contains a bromodomain able to bind histones H3 and H4. We propose that this array of domains constitute a nucleosome interaction module that helps BRM to interact with its substrate. We also characterize an Arabidopsis mutant that expresses a BRM protein lacking the last 454 amino acid residues (BRM-AC), encompassing the bromodomain and two of the three DNA-binding activities identified. This mutant displays an intermediate phenotype between those of the wild-type and a null allele mutant, suggesting that the nucleosome interaction module is required for the normal function of BRM but it is not essential for the remodelling activity of BRM-containing SWI/SNF complexes. (C) 2007 Elsevier Ltd. All rights reserved.
引用
收藏
页码:240 / 250
页数:11
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