The phasevarion: A genetic system controlling coordinated, random switching of expression of multiple genes

被引：144

作者：

Srikhanta, YN

Maguire, TL

Stacey, KJ

Grimmond, SM

Jennings, MP ^{[1
]}

机构：

[1] Univ Queensland, Sch Mol & Microbial Sci, Brisbane, Qld 4072, Australia

[2] Univ Queensland, Inst Mol Biosci, Brisbane, Qld 4072, Australia

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2005年 / 102卷 / 15期

关键词：

DNA methyltransferase; Haemophilus influenzae; phase variation;

D O I：

10.1073/pnas.0501169102

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Several host-adapted bacterial pathogens contain methyltransferases associated with type III restriction-modification (R-M) systems that are subject to reversible, high-frequency on/off switching of expression (phase variation). To investigate the role of phase-variable expression of R-M systems, we made a mutant strain lacking the methyltransferase (mod) associated with a type III R-M system of Haemophilus influenzae and analyzed its phenotype. By microarray analysis, we identified a number of genes that were either up- or down-regulated in the mod mutant strain. This system reports the coordinated random switching of a set of genes in a bacterial pathogen and may represent a widely used mechanism.

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页码：5547 / 5551

页数：5

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