Crystallization and preliminary X-ray analysis of high-alkaline pectate lyase

Pel-15, a high-alkaline pectate lyase (pectate transeliminase; E.C. 4.2.2.2) from Bacillus sp. strain KSM-P15, has been crystallized using the hanging-drop vapour-diffusion method at 277 K. Two different crystal forms were obtained and preliminary X-ray diffraction data were collected from each crystal form at 100 K. Both forms belong to the orthorhombic space group P2(1)2(1)2(1) and contain one molecule per asymmetric unit. The unit-cell parameters of form I are a = 43.2 (2), b = 60.2 (2), c = 82.2 (2) Angstrom and those of form II are a = 42.9 (1), b = 43.4 (1), c = 105.9 (3) Angstrom. Diffraction data to a resolution of 1.5 Angstrom were collected from form II crystals using a synchrotron-radiation source.