GSH-dependent peroxidase activity of the rice (Oryza sativa) glutaredoxin, a thioltransferase

被引：36

作者：

Lee, KO ^{[1
]}

Lee, JR ^{[1
]}

Yoo, JY ^{[1
]}

Jang, HH ^{[1
]}

Moon, JC ^{[1
]}

Jung, BG ^{[1
]}

Chi, YH ^{[1
]}

Park, SK ^{[1
]}

Lee, SS ^{[1
]}

Lim, CO ^{[1
]}

Yun, DJ ^{[1
]}

Cho, MJ ^{[1
]}

Lee, SY ^{[1
]}

机构：

[1] Gyeongsang Natl Univ, BMBBRC, Dept Biochem, Div Appl Life Sci,BK21 Program, Chinju 660701, South Korea

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2002年 / 296卷 / 05期

关键词：

dual function; GSH-dependent peroxidase; rice; glutaredoxin; thioltransferase;

D O I：

10.1016/S0006-291X(02)02047-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Glutaredoxin (Grx) is a 12-kDa thioltransferase that reduces disulfide bonds of other proteins and maintains the redox potential cells. In addition to its oxidoreductase activity, we report here that a rice Grx (OsGrx) can also function as a GSH-dependent peroxidase. Because of this antioxidant activity, OsGrx protects glutamine synthetase from oxidative damage. Individually replacing the conserved Cys residues in OsGrx with Ser shows that Cys(26), but not Cys(26), is essential for the thioltransferase and GSH-dependent peroxidase activities. Kinetic characterization of OsGrx reveals that the maximal catalytic efficiency (V-max/K-m) is obtained with cumene hydroperoxide rather than H2O2 or t-butyl hydroperoxide. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：1152 / 1156

页数：5

共 26 条

[1] 1-Cys peroxiredoxin, a bifunctional enzyme with glutathione peroxidase and phospholipase A2 activities [J].