A metabonomic analysis of plasma from Zucker rat strains using gas chromatography/mass spectrometry and pattern recognition

被引：78

作者：

Major, Hilary J.

Williams, Rebecca

Wilson, Amy J.

Wilson, Ian D.

机构：

[1] Waters Corp, Manchester M22 5PP, Lancs, England

[2] AstraZeneca, Dept Drug Metab & Pharmacokinet, Macclesfield SK10 4TG, Cheshire, England

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2006年 / 20卷 / 22期

关键词：

D O I：

10.1002/rcm.2732

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Plasma obtained from three strains of Zucker rats was analysed using capillary gas chromatography/ mass spectrometry (GC/MS) to obtain global metabolite profiles as part of a series of metabonomic investigations of animal models of diabetes. Samples were obtained from 20-week-old male wild-type Zucker lean, (fa/fa) obese and lean/(fa) animals and were analysed following protein precipitation, using acetonitrile, and derivatisation. Subsequent data analysis using principal components analysis (PCA) and orthogonal projection to latent structures (OPLS) revealed differences between the plasma metabolite profiles of the three strains, with those of the Zucker lean and the leanl(fa) crosses being similar to each other whilst differing from the (fa/fa) obese strain. Copyright (c) 2006 John Wiley & Sons, Ltd.

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页码：3295 / 3302

页数：8

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