Cytomegalovirus DNA Quantification Using an Automated Platform for Nucleic Acid Extraction and Real-Time PCR Assay Setup

被引：13

作者：

Forman, Michael ^{[1
]}

Wilson, Andy ^{[2
]}

Valsamakis, Alexandra ^{[1
]}

机构：

[1] Johns Hopkins Med Inst, Div Microbiol, Baltimore, MD 21205 USA

[2] ARUP Inst Clin & Expt Pathol, ARUP Labs, Salt Lake City, UT USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2011年 / 49卷 / 07期

关键词：

D O I：

10.1128/JCM.00721-11

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Analytical performance characteristics of the QIAsymphony RGQ system with artus cytomegalovirus ( CMV) reagents were determined. Measurable range spanned 2.0 to >= 7.0 log(10) copies/ml. The detection limit was 23 copies/ml. Intrarun and interrun coefficients of variation were <= 2.1% at 3.0 and 5.0 log(10) copies/ml. In clinical specimens, RGQ values were similar to 0.2 log(10) copies/ml higher than those in an assay using a BioRobot M48 extraction/manual reaction setup/7500 Real-Time PCR instrument. No cross-contamination was observed.

引用

页码：2703 / 2705

页数：3

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