Arginine-serine-rich domains bound at splicing enhancers contact the branchpoint to promote prespliceosome assembly
被引:196
作者:
Shen, HH
论文数: 0引用数: 0
h-index: 0
机构:Univ Massachusetts, Sch Med, Howard Hughes Med Inst, Program Gene Funct & Express, Worcester, MA 01605 USA
Shen, HH
Kan, JLC
论文数: 0引用数: 0
h-index: 0
机构:Univ Massachusetts, Sch Med, Howard Hughes Med Inst, Program Gene Funct & Express, Worcester, MA 01605 USA
Kan, JLC
Green, MR
论文数: 0引用数: 0
h-index: 0
机构:
Univ Massachusetts, Sch Med, Howard Hughes Med Inst, Program Gene Funct & Express, Worcester, MA 01605 USAUniv Massachusetts, Sch Med, Howard Hughes Med Inst, Program Gene Funct & Express, Worcester, MA 01605 USA
Green, MR
[1
]
机构:
[1] Univ Massachusetts, Sch Med, Howard Hughes Med Inst, Program Gene Funct & Express, Worcester, MA 01605 USA
[2] Univ Massachusetts, Sch Med, Program Mol Med, Worcester, MA 01605 USA
Exonic splicing enhancers (ESEs) are required for splicing of certain pre-mRNAs and function by providing binding sites for serine-arginine (SR) proteins, which contain an arginine-serine-rich (RS) domain. How an RS domain bound at the ESE promotes splicing is poorly understood. We have developed an RNA-protein crosslinking procedure to identify the target of the ESE-bound RS domain. Using this approach, we show that the ESE-bound RS domain specifically contacts the pre-mRNA branchpoint. The interaction between the ESE-bound RS domain and the branchpoint occurs in the prespliceosome and is dependent upon the same splicing signals, biochemical factors, and reaction conditions required to support prespliceosome assembly. Analysis of RS domain mutants demonstrates that the ability to interact with the branchpoint, to promote prespliceosome assembly, and to support splicing are related activities. We conclude that the ESE-bound RS domain functions by contacting the branchpoint to promote prespliceosome assembly.
机构:
Univ Calif Los Angeles, Howard Hughes Med Inst, Dept Microbiol Immunol & Mol Genet, Los Angeles, CA 90095 USAUniv Calif Los Angeles, Howard Hughes Med Inst, Dept Microbiol Immunol & Mol Genet, Los Angeles, CA 90095 USA
机构:
Univ Toronto, Charles H Best Inst, Dept Med Res, Toronto, ON M5G 1L6, CanadaUniv Toronto, Charles H Best Inst, Dept Med Res, Toronto, ON M5G 1L6, Canada
机构:
COLD SPRING HARBOR LAB, POB 100, 1 BUNGTOWN RD, COLD SPRING HARBOR, NY 11724 USACOLD SPRING HARBOR LAB, POB 100, 1 BUNGTOWN RD, COLD SPRING HARBOR, NY 11724 USA
CACERES, JF
;
KRAINER, AR
论文数: 0引用数: 0
h-index: 0
机构:
COLD SPRING HARBOR LAB, POB 100, 1 BUNGTOWN RD, COLD SPRING HARBOR, NY 11724 USACOLD SPRING HARBOR LAB, POB 100, 1 BUNGTOWN RD, COLD SPRING HARBOR, NY 11724 USA
机构:
Univ Calif Los Angeles, Howard Hughes Med Inst, Dept Microbiol Immunol & Mol Genet, Los Angeles, CA 90095 USAUniv Calif Los Angeles, Howard Hughes Med Inst, Dept Microbiol Immunol & Mol Genet, Los Angeles, CA 90095 USA
机构:
Univ Toronto, Charles H Best Inst, Dept Med Res, Toronto, ON M5G 1L6, CanadaUniv Toronto, Charles H Best Inst, Dept Med Res, Toronto, ON M5G 1L6, Canada
机构:
COLD SPRING HARBOR LAB, POB 100, 1 BUNGTOWN RD, COLD SPRING HARBOR, NY 11724 USACOLD SPRING HARBOR LAB, POB 100, 1 BUNGTOWN RD, COLD SPRING HARBOR, NY 11724 USA
CACERES, JF
;
KRAINER, AR
论文数: 0引用数: 0
h-index: 0
机构:
COLD SPRING HARBOR LAB, POB 100, 1 BUNGTOWN RD, COLD SPRING HARBOR, NY 11724 USACOLD SPRING HARBOR LAB, POB 100, 1 BUNGTOWN RD, COLD SPRING HARBOR, NY 11724 USA