Cytokinin stimulates and abscisic acid inhibits greening of etiolated Lupinus luteus cotyledons by affecting the expression of the light-sensitive protochlorophyllide oxidoreductase

被引:47
作者
Kusnetsov, V
Herrmann, RG
Kulaeva, ON
Oelmüller, R
机构
[1] Univ Jena, Inst Allgemeine Bot, Lehrstuhl Pflanzenphysiol, D-07743 Jena, Germany
[2] Univ Munich, Inst Bot, D-80638 Munich, Germany
[3] Russian Acad Sci, KA Timiryazev Plant Physiol Inst, Moscow 127276, Russia
来源
MOLECULAR AND GENERAL GENETICS | 1998年 / 259卷 / 01期
关键词
abscisic acid; cytokinin; plastid biogenesis; protochlorophyllide oxidoreductase;
D O I
10.1007/s004380050784
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Plastid biogenesis in etiolated lupine (Lupinus luteus L.) cotyledons is highly sensitive to cytokinins and abscisic acid. In the presence of the synthetic cytokinin N-6-benzylaminopurine, greening and plastid biogenesis is substantially promoted as compared to untreated controls, whereas abscisic acid has an inhibitory effect. Faster greening in cytokinin-treated cotyledons is accompanied by a higher level and slower degradation of the light-sensitive protochlorophyllide-oxidoreductase (POR); while ABA has the opposite effect. The phytohormones appear to modulate FOR gene expression, since the steady-state levels of FOR mRNA, as well as transcripts of other nuclear genes for plastid proteins, are strongly increased by cytokinin and reduced by abscisic acid treatment. When etiolated lupine cotyledons were illuminated with far-red light prior to phytohormone application, the FOR level substantially decreased; this was accompanied by the loss of the phytohormone's effect on greening. Based on these findings it is concluded that the level of FOR and the integrity of the prolamellar body is crucial for cytokinin- and abscisic acid-controlled greening following transfer of etiolated lupine cotyledons into the light.
引用
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页码:21 / 28
页数:8
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