At least two origins of fungicide resistance in grapevine downy mildew Populations

被引:122
作者
Chen, Wei-Jen
Delmotte, Francois
Richard-Cervera, Sylvie
Douence, Lisette
Greif, Charles
Corio-Costet, Marie-France
机构
[1] Inst Natl Rech Agron, UMR Sante Vegetale, INRA ENITAB, Inst Sci Vigne & Vin, F-33883 Villenave Dornon, France
[2] Inst Natl Rech Agron, UMR Sante Vigne & Qual Vin, INRA ULP, F-68021 Colmar, France
关键词
D O I
10.1128/AEM.00507-07
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Quinone outside inhibiting (QoI) fungicides represent one of the most widely used groups of fungicides used to control agriculturally important fungal pathogens. They inhibit the cytochrome be, complex of mitochondrial respiration. Soon after their introduction onto the market in 1996, QoI fungicide- resistant isolates were detected in field plant pathogen populations of a large range of species. However, there is still little understanding of the processes driving the development of QoI fungicide resistance in plant pathogens. In particular, it is unknown whether fungicide resistance occurs independently in isolated populations or if it appears once and then spreads globally by migration. Here, we provide the first case study of the evolutionary processes that lead to the emergence of QoI fungicide resistance in the plant pathogen Plasmopara viticola. Sequence analysis of the complete cytochrome b gene showed that all resistant isolates carried a mutation resulting in the replacement of glycine by alanine at codon 143 (G143A). Phylogenetic analysis of a large mitochondrial DNA fragment including the cytochrome b gene (2,281 bp) across a wide range of European P. viticola isolates allowed the detection of four major haplotypes belonging to two distinct clades, each of which contains a different QoI fungicide resistance allele. This is the first demonstration that a selected substitution conferring resistance to a fungicide has occurred several times in a plant-pathogen system. Finally, a high population structure was found when the frequency of QoI fungicide resistance haplotypes was assessed in 17 French vineyards, indicating that pathogen populations might be under strong directional selection for local adaptation to fungicide pressure.
引用
收藏
页码:5162 / 5172
页数:11
相关论文
共 62 条
[1]   Multiple origins of pyrethroid resistance in sympatric biotypes of Bemisia tabaci (Hemiptera: Aleyrodidae) [J].
Alon, M ;
Benting, J ;
Lueke, B ;
Ponge, T ;
Alon, F ;
Morin, S .
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2006, 36 (01) :71-79
[2]   Multiple origins of cyclodiene insecticide resistance in Tribolium castaneum (Coleoptera: Tenebrionidae) [J].
Andreev, D ;
Kreitman, M ;
Phillips, TW ;
Beeman, RW ;
ffrench-Constant, RH .
JOURNAL OF MOLECULAR EVOLUTION, 1999, 48 (05) :615-624
[3]  
[Anonymous], 1998, EVOLUTIONARY GENETIC
[4]   Evidence for multiple origins of identical insecticide resistance mutations in the aphid Myzus persicae [J].
Anstead, JA ;
Williamson, MS ;
Denholm, I .
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2005, 35 (03) :249-256
[5]   MOLECULAR ANALYSIS OF CYCLODIENE RESISTANCE-ASSOCIATED MUTATIONS AMONG POPULATIONS OF THE SWEET-POTATO WHITEFLY BEMISIA-TABACI [J].
ANTHONY, NM ;
BROWN, JK ;
MARKHAM, PG ;
FFRENCHCONSTANT, RH .
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, 1995, 51 (03) :220-228
[6]   Characterization of spontaneous mutants of Magnaporthe grisea expressing stable resistance to the Qo-inhibiting fungicide azoxystrobin [J].
Avila-Adame, C ;
Köller, W .
CURRENT GENETICS, 2003, 42 (06) :332-338
[7]   The population biology of mitochondrial DNA and its phylogenetic implications [J].
Ballard, JWO ;
Rand, DM .
ANNUAL REVIEW OF ECOLOGY EVOLUTION AND SYSTEMATICS, 2005, 36 :621-642
[8]   Median-joining networks for inferring intraspecific phylogenies [J].
Bandelt, HJ ;
Forster, P ;
Röhl, A .
MOLECULAR BIOLOGY AND EVOLUTION, 1999, 16 (01) :37-48
[9]  
Barlow M, 2003, GENETICS, V163, P1237
[10]   The strobilurin fungicides [J].
Bartlett, DW ;
Clough, JM ;
Godwin, JR ;
Hall, AA ;
Hamer, M ;
Parr-Dobrzanski, B .
PEST MANAGEMENT SCIENCE, 2002, 58 (07) :649-662