Polyethylenimine-functionalized graphene oxide as an efficient gene delivery vector

被引:263
作者
Chen, Biao [1 ,2 ]
Liu, Min [2 ]
Zhang, Liming [2 ]
Huang, Jie [2 ]
Yao, Jianlin [1 ]
Zhang, Zhijun [2 ]
机构
[1] Soochow Univ, Coll Chem Chem Engn & Mat Sci, Suzhou 215123, Peoples R China
[2] Chinese Acad Sci, Div Nanobiomed, Suzhou Inst Nanotech & Nanobion, Suzhou 215123, Peoples R China
关键词
DNA; NANOPARTICLES; THERAPY; DRUGS;
D O I
10.1039/c1jm10341e
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
A novel gene delivery system based on graphene oxide chemicallyfunctionalized with branched polyethylenimine (PEI-GO) is reported. The PEI-GO conjugate was formed by the covalent linking of PEI and GO via an amide bond by widely used EDC chemistry. Thus prepared PEI-GO exhibits an excellent ability to condense DNA at a low mass ratio with a positive potential of 49 mV. A WST assay reveals that PEI-GO is significantly less cytotoxic than PEI 25 kDa. Finally, the transfection efficiency of PEI-GO was evaluated. It is demonstrated that the luciferase expression of PEIGO is comparable or even higher than that of the PEI 25 kDa at optimal mass ratio. Moreover, intracellular tracking of Cy3-labelled pGL-3 indicates that PEI-GO could effectively deliver plasmid DNA into cells and be localized in the nucleus. These findings suggest that PEI-GO is a promising candidate for efficient gene delivery.
引用
收藏
页码:7736 / 7741
页数:6
相关论文
共 24 条
[1]   pH-sensitive cationic polymer gene delivery vehicle:: N-Ac-poly(L-histidine)-graft-poly(L-lysine) comb shaped polymer [J].
Benns, JM ;
Choi, JS ;
Mahato, RI ;
Park, JS ;
Kim, SW .
BIOCONJUGATE CHEMISTRY, 2000, 11 (05) :637-645
[2]  
Chen Yuxiang, 2003, Current Gene Therapy, V3, P273, DOI 10.2174/1566523034578339
[3]   Natural polymers for gene delivery and tissue engineering [J].
Dang, Jiyoung M. ;
Leong, Kam W. .
ADVANCED DRUG DELIVERY REVIEWS, 2006, 58 (04) :487-499
[4]   Factors affecting blood clearance and in vivo distribution of polyelectrolyte complexes for gene delivery [J].
Dash, PR ;
Read, ML ;
Barrett, LB ;
Wolfert, M ;
Seymour, LW .
GENE THERAPY, 1999, 6 (04) :643-650
[5]  
Dominik E. D., 2009, ADV DRUG DELIVERY RE, V61, P554
[6]   PREPARATION OF GRAPHITIC OXIDE [J].
HUMMERS, WS ;
OFFEMAN, RE .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1958, 80 (06) :1339-1339
[7]   Layer-by-layer assembly of ultrathin composite films from micron-sized graphite oxide sheets and polycations [J].
Kovtyukhova, NI ;
Ollivier, PJ ;
Martin, BR ;
Mallouk, TE ;
Chizhik, SA ;
Buzaneva, EV ;
Gorchinskiy, AD .
CHEMISTRY OF MATERIALS, 1999, 11 (03) :771-778
[8]   Lipid-based nanoparticles for nucleic acid delivery [J].
Li, Weijun ;
Szoka, Francis C., Jr. .
PHARMACEUTICAL RESEARCH, 2007, 24 (03) :438-449
[9]   Polyethylenimine-grafted multiwalled carbon nanotubes for secure noncovalent immobilization and efficient delivery of DNA [J].
Liu, Y ;
Wu, DC ;
Zhang, WD ;
Jiang, X ;
He, CB ;
Chung, TS ;
Goh, SH ;
Leong, KW .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2005, 44 (30) :4782-4785
[10]   PEGylated nanographene oxide for delivery of water-insoluble cancer drugs [J].
Liu, Zhuang ;
Robinson, Joshua T. ;
Sun, Xiaoming ;
Dai, Hongjie .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 130 (33) :10876-+