Improvement of arbuscular mycorrhiza development by inoculation of soil with phosphate-solubilizing rhizobacteria to improve rock phosphate bioavailability (P-32) and nutrient cycling

被引:196
作者
Toro, M [1 ]
Azcon, R [1 ]
Barea, JM [1 ]
机构
[1] CSIC, ESTAC EXPT ZAIDIN, DEPT MICROBIOL SUELO & SISTEMAS SIMBIOT, GRANADA 18008, SPAIN
关键词
D O I
10.1128/AEM.63.11.4408-4412.1997
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The interactive effect of phosphate-solubilizing bacteria and arbuscular mycorrhizal (AM) fungi on plant use of soil P sources of low bioavailability (endogenous or added as rock phosphate [RP] material) was evaluated by using soil microcosms which integrated P-32 isotopic dilution techniques. The microbial inocula consisted of the AM fungus Glomus intraradices and two phosphate-solubilizing rhizobacterial isolates: Enterobacter sp, and Bacillus subtilis, These rhizobacteria behaved as ''mycorrhiza helper bacteria'' promoting establishment of both the indigenous and the introduced AM endophytes despite a gradual decrease in bacterial population size, which dropped from 10(7) at planting to 10(3) CFU g(-1) of dry rhizosphere soil at harvest. Dual inoculation with G. intraradices and B. subtilis significantly increased biomass and N and P accumulation in plant tissues. Regardless of the rhizobacterium strain and of the addition of RP, AM: plants displayed lower specific activity (P-32/P-31) than their comparable controls, suggesting that the plants used P sources not available in their absence, The inoculated rhizobacteria may have released phosphate ions (P-31), either from the added RP or from the less-available indigenous P sources, which were effectively taken up by the external AM mycelium, Soluble Ca deficiency in the test soil may have benefited P solubilization. At least 75% of the P in dually inoculated plants derived from the added RP, It appears that these mycorrhizosphere interactions between bacterial and fungal plant associates contributed to the biogeochemical P cycling, thus promoting a sustainable nutrient supply to plants.
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页码:4408 / 4412
页数:5
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