Complex FISH probes for the subtelomeric regions of all human chromosomes: comparative hybridization of CEPH YACs to chromosomes of the Old World monkey Presbytis cristata and great apes

被引：38

作者：

Kingsley, K ^{[1
]}

Wirth, J ^{[1
]}

vanderMaarel, S ^{[1
]}

Freier, S ^{[1
]}

Ropers, HH ^{[1
]}

Haaf, T ^{[1
]}

机构：

[1] MAX PLANCK INST MOL GENET,D-14195 BERLIN,GERMANY

来源：

CYTOGENETICS AND CELL GENETICS | 1997年 / 78卷 / 01期

关键词：

D O I：

10.1159/000134616

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We have generated a human subtelomere probe panel, utilizing well characterized CEPH YACs, for the investigation of human chromosome pathology and evolution through fluorescent in situ hybridization (FISH). Region-specific FISH probes will be extremely valuable for detecting cytogenetically cryptic telomere abnormalities. Here, we present the first comparative mapping study (with 29 subtelomere probes and 6 chromosome paints) to the Old World monkey Presbytis cristata, followed by hybridizations to the great apes, gorilla and orangutan, when rearrangements were detected. We observed that the position of telomere-associated genomic sequences has been only moderately conserved during primate evolution. YAC 364f9, specific for the subtelomeric long arm of human chromosome 3, contains an evolutionary inversion breakpoint that was involved in independent chromosome rearrangements in P. cristata and gorilla.

引用

页码：12 / 19

页数：8

共 27 条

[1] Integration of the cytogenetic, genetic, and physical maps of the human genome by FISH mapping of CEPH YAC clones [J].