Purification and crystallization of CII:: An unstable transcription activator from phage λ

被引：9

作者：

Datta, AB

Chakrabarti, P

Subramanya, HS

Parrack, P

机构：

[1] Bose Inst, Dept Biochem, Kolkata 700054, India

[2] Cent Drug Res Inst, Mol & Struct Biol Div, Lucknow 226001, Uttar Pradesh, India

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2001年 / 288卷 / 04期

关键词：

bacteriophage lambda; CII protein; crystallization; lysogeny; genetic switch;

D O I：

10.1006/bbrc.2001.5880

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The CII protein of the temperate bacteriophage lambda is a transcriptional activator involved in the lysis-lysogeny switch of the phage. It is an unstable protein of 97 amino acids and is known to exist as a tetramer in the native state. The cII gene has been cloned and expressed in Escherichia coli using a T7 promoter based over-expression system. The recombinant CII protein has been purified to homogeneity by ammonium sulfate fractionation followed by two steps of ion-exchange chromatography. The purified protein crystallized at pH 8.2 in hanging-drop vapor diffusion method at 293 K. The crystals diffract to a resolution of 2.8 Angstrom and belong to the space group C222 with unit-cell parameters a = 64.10, b = 106.95 and c = 120.16 Angstrom. (C) 2001 Academic Press.

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收藏

页码：997 / 1000

页数：4

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