Selection of RNA aptamers against human influenza virus hemagglutinin using surface plasmon resonance

被引：148

作者：

Misono, TS

Kumar, PKR

机构：

[1] Natl Inst Adv Ind Sci & Technol, Inst Biol Resources & Funct, Tsukuba, Ibaraki 3058566, Japan

[2] Univ Tsukuba, Inst Biol Sci, Tsukuba, Ibaraki 3058577, Japan

来源：

ANALYTICAL BIOCHEMISTRY | 2005年 / 342卷 / 02期

关键词：

aptamer; surface plasmon resonance; in vitro selection; hemagglutinin;

D O I：

10.1016/j.ab.2005.04.013

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Aptamers are functional nucleic acids possessing high affinity and specificity to their cognate ligands and are isolated from a library of nucleic acids by iterative rounds of selection and amplification. In the current study.. we used surface plasmon resonance (Biacore) as an efficient methodology for selecting aptamers that bind to hemagglutinin (HA) of human influenza virus. This procedure allowed us to monitor and select the target-bound aptamers specifically and simultaneously. These studies not only yielded an aptamer that binds to the HA of influenza virus with high affinity but also revealed the consensus sequence, 5'-GUCGNCNU(N)(2-3)GUA-3, for HA recognition. (c) 2005 Elsevier Inc. All rights reserved.

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页码：312 / 317

页数：6

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