Oct4 dependence of chromatin structure within the extended Nanog locus in ES cells

被引：94

作者：

Levasseur, Dana N. ^{[1
,2
]}

Wang, Jianlong ^{[1
,2
]}

Dorschner, Michael O. ^{[3
]}

Stamatoyannopoulos, John A. ^{[3
]}

Orkin, Stuart H. ^{[1
,2
,4
]}

机构：

[1] Harvard Univ, Sch Med, Dana Farber Canc Inst, Harvard Stem Cell Inst, Boston, MA 02115 USA

[2] Childrens Hosp, Div Hematol Oncol, Boston, MA 02115 USA

[3] Univ Washington, Dept Genome Sci, Seattle, WA 98195 USA

[4] Howard Hughes Med Inst, Boston, MA 02115 USA

来源：

GENES & DEVELOPMENT | 2008年 / 22卷 / 05期

关键词：

Nanog; Oct4; chromatin; reprogramming; self renewal;

D O I：

10.1101/gad.1606308

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Embryonic stem (ES) cells offer insight into early developmental fate decisions, and their controlled differentiation may yield vast regenerative potential. The molecular determinants supporting ES cell self-renewal are incompletely understood. The homeodomain proteins Nanog and Oct4 are essential for mouse ES cell self-renewal. Using a high-throughput approach, we discovered DNaseI hypersensitive sites and potential regulatory elements along a 160-kb region of the genome that includes GDF3, Dppa3, and Nanog. We analyzed gene expression, chromatin occupancy, and higher-order chromatin structure throughout this gene locus and found that expression of the reprogramming factor Oct4 is required to maintain its integrity.

引用

页码：575 / 580

页数：6

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