Integrated compact biocompatible hydrogel-based amperometric sensing device for easy screening of drugs involved in nitric oxide production by adherent cultured cells

被引：11

作者：

Wartelle, C

Schuhmann, W

Blöchl, A

Bedioui, F

机构：

[1] Ecole Natl Super Chim Paris, Lab Pharmcol Chim & Genet, CNRS,UMR 8151, INSERM,U640, F-75231 Paris, France

[2] Ruhr Univ Bochum, Lehrstuhl Mol Neurobiochem, D-44780 Bochum, Germany

来源：

ELECTROCHIMICA ACTA | 2005年 / 50卷 / 25-26期

关键词：

biosensor; nitric oxide; hydrogel; adherent cells; compact device;

D O I：

10.1016/j.electacta.2004.12.048

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

We report in this study on the first use a compact biocompatible hydrogel-based electrochemical sensing device for the examination of NO production from adherently growing cells. The conception assessment is tested here in the case of C6 glioma cells. The main body of the integrated device is made of three-planar disc (1 mm of diameter each) array electrode with a mounted acrylic reservoir, and the resulting chamber served as the electrochemical cell. The ionically conducting biocompatible hydrogel membrane is then deposited on the planar surface of the substrate on which the examined NO-releasing cells are cultured. Such a device allows having a living cell-based device for an easy screening of drugs for NO production and/or inhibition. (C) 2005 Elsevier Ltd. All rights reserved.

引用

页码：4988 / 4994

页数：7

共 17 条

[1] Elaboration and use of nickel planar macrocyclic complex-based sensors for the direct electrochemical measurement of nitric oxide in biological media [J].