Rapid detection of enterovirus 71 by real-time TaqMan RT-PCR

被引：94

作者：

Tan, Eng Lee ^{[1
,2
]}

Yong, Li Li Gaynor ^{[1
]}

Quak, Seng Hock ^{[3
]}

Yeo, Wei Cheng Andrea ^{[2
]}

Chow, Vincent Tak Kwong ^{[1
]}

Poh, Chit Laa ^{[1
,4
]}

机构：

[1] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Microbiol, Singapore 117597, Singapore

[2] Singapore Polytech, Sch Chem & Life Sci, Singapore 139651, Singapore

[3] Natl Univ Singapore Hosp, Dept Pediat, Singapore 119074, Singapore

[4] Swinburne Univ Technol, Fac Life & Social Sci, Environm & Biotechnol Ctr, Hawthorn, Vic 3122, Australia

来源：

JOURNAL OF CLINICAL VIROLOGY | 2008年 / 42卷 / 02期

关键词：

hand; foot and mouth disease (HFMD); enterovirus; 71; real-time TaqMan RT-PCR;

D O I：

10.1016/j.jcv.2008.01.001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Background: Enterovirus 71 (EV71) is the main etiological agent of Hand, Foot and Mouth Disease (HFMD) and has been associated with neurological complications which resulted in fatalities during recent outbreaks in Asia Pacific region. Objective: Develop a real-time TaqMan RT-PCR for rapid detection of EV71. Study design: Specific primers and probe were designed based on highly conserved VP1 region of EV71. The sensitivity of the real-time RT-PCR was evaluated with 67 clinical specimens collected from pediatric patients with suspected HFMD. Results: Our real-time TaqMan RT-PCR showed 100% specificity in detecting EV71 and showed an analytical sensitivity of 5 viral copies. High sensitivity was also achieved in detecting EV71 directly from clinical specimens. Conclusions: Real-time TaqMan RT-PCR offers a rapid and sensitive method to detect EV71 from clinical specimens, and will allow quarantine measures to be taken more effectively during outbreaks. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：203 / 206

页数：4

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