Nucleophosmin/B23-binding peptide inhibits tumor growth and up-regulates transcriptional activity of p53

被引：25

作者：

Chan, HJ

Weng, JJ

Yung, BYM ^{[1
]}

机构：

[1] Chang Gung Univ, Coll Med, Chang Gung Genom Res Ctr, Taoyuan 333, Taiwan

[2] Chang Gung Univ, Coll Med, Dept Pharmacol, Taoyuan 333, Taiwan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 333卷 / 02期

关键词：

nucleophosmin/B23; Rev peptide; p53;

D O I：

10.1016/j.bbrc.2005.04.176

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Rev peptide that binds to nucleophosmin/B23 with the highest affinity exhibited the greatest cytotoxicity on Ras-3T3 cells and inhibited tumor growth most effectively in nude mice. The efficiency of colony formation in soft agar of Ras-3T3 cells was significantly inhibited by treatment with Rev peptide. In addition, Rev peptide could potentiate the doxorubicin-induced decrease of cellular viability in U1 bladder cancer cells and inhibition of tumor growth in nude mice. Treatment of Rev peptide increased protein expression and transcriptional activity of p53 and inhibited the nucleophosmin/B23-mediated PCNA promoter activation. Peptides having high affinity of binding to molecular targets such as nucleophosmin/B23 represent a potentially useful approach to anti-cancer biotherapeutics. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：396 / 403

页数：8

共 38 条

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