Ambient air particulate matter exposure and tissue factor expression in atherosclerosis

被引:35
作者
Sun, Qinghua [3 ,4 ]
Yue, Peibin
Kirk, Rita I. [5 ]
Wang, Aixia
Moatti, Didier [6 ]
Jin, Ximei [1 ,7 ]
Lu, Bo [8 ]
Schecter, Alison D. [5 ]
Lippmann, Morton [1 ,2 ]
Gordon, Terry [1 ,2 ]
Chen, Lung Chi [1 ,2 ]
Rajagopalan, Sanjay
机构
[1] NYU, Dept Environm Med, Sch Med, Tuxedo Pk, NY 10987 USA
[2] NYU, Sch Med, Nelson Inst Environm Med, Tuxedo Pk, NY 10987 USA
[3] Ohio State Univ, Coll Med & Publ Hlth, Div Environm Hlth Sci, Columbus, OH 43210 USA
[4] Ohio State Univ, Coll Med & Publ Hlth, Davis Heart & Lung Res Inst, Columbus, OH 43210 USA
[5] Mt Sinai Sch Med, Cardiovasc Inst, New York, NY USA
[6] NYU, Sch Med, Mt Sinai Sch Med, Cardiovasc Inst, Tuxedo Pk, NY USA
[7] Nelson Inst Environm Med, New York, NY USA
[8] Ohio State Univ, Coll Publ Hlth, Div Biostat, Columbus, OH 43210 USA
关键词
D O I
10.1080/08958370701821482
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 [卫生毒理学];
摘要
Recent studies have suggested a link between inhaled particulate matter (PM) exposure and atherogenesis. We investigated tissue factor (TF) expression with ambient fine particulate matter (diameter <2.5 mu m, PM2.5) exposure and in response to in vitro exposure to fine and ultrafine PM in cultured human bronchial epithelial cells, vascular smooth muscle cells (hSMCs), and monocytes. ApoE(-/-) mice, fed with normal chow (NC) or high-fat chow (HFC), were exposed to concentrated PM2.5 or filtered air (FA) for 6 mo (6 h/day, 5 day/wk, n = 28). Following in vivo ultrasound bio-microscopy (UBM) assessment of plaque area, macrophage infiltration (CD68) and TF expression in the aorta were quantified. Cultured cells were incubated with size-fractionated PM from cascade impactors, or with standard reference PM material (SRM, number 1649a) and assayed for TF protein, mRNA, and activity. UBM-derived plaque areas were 7 +/- 1% larger in the PM2.5-HFC than the FA-HFC group (p = .04), but not significantly different between the PM2.5-NC and FA-NC groups (p = .07). Immunohistochemistry revealed increased TF (15 +/- 3% vs. 8 +/- 2%, p < .01) and macrophage infiltration (19 +/- 2% vs. 14 +/- 3%, p < .01) in the plaques of PM2.5-HFC compared with FA-HFC groups. Impactor-collected PM2.5 and ultrafine particles consistently increased TF protein in bronchial epithelial cells, monocytes, and hSMCs. TF mRNA expression increased rapidly (within 1 h) in response to SRM PM. We conclude that in vivo and in vitro exposure to ambient air PM2.5 induces TF expression.
引用
收藏
页码:127 / 137
页数:11
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