DNA loop domains in a 1.4-Mb region around the human hprt gene mapped by cleavage mediated by nuclear matrix-associated topoisomerase II

被引:8
作者
Fajkus, J
Nicklas, JA
Hancock, R
机构
[1] Univ Laval, Ctr Rech Cancerol, Hotel Dieu, Quebec City, PQ G1R 2J6, Canada
[2] Acad Sci Czech Republ, Inst Biophys, CS-61265 Brno, Czech Republic
[3] Univ Vermont, VCC Genet Lab, Burlington, VT 05401 USA
来源
MOLECULAR AND GENERAL GENETICS | 1998年 / 260卷 / 05期
关键词
DNA loop domains; human hprt gene; topoisomerase II; nuclear matrix;
D O I
10.1007/s004380050911
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have mapped the positions in a similar to 1.4-Mb region of genomic DNA around the human hprt gene which are accessible in vivo to cleavage by topoisomerase II associated with the nuclear matrix. These positions, which are interpreted as the boundaries of DNA loop domains, were mapped in K562 cells by examining the truncation of rare-cutter restriction fragments separated by pulsed field gel electrophoresis after topoisomerase II-mediated cleavage, using seven linked markers mapped in this region as probes for indirect end-labeling. Eleven cleavage positions were detected and were interpreted as defining ten loop domains of lengths between 70 and 210 kb (average similar to 135 kb); the hprt gene resides in a 150-kb loop domain. Loop domain boundaries coincided with three of the fifteen deletion breakpoints mapped in a 600-kb sector of this region in human lymphocytes, within the limits of resolution of pulsed field gel electrophoresis; this correlation was not statistically significant.
引用
收藏
页码:410 / 416
页数:7
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