Francisella tularensis strain typing using multiple-locus, variable-number tandem repeat analysis

被引:121
作者
Farlow, J
Smith, KL
Wong, J
Abrams, M
Lytle, M
Keim, P
机构
[1] No Arizona Univ, Dept Biol Sci, Flagstaff, AZ 86011 USA
[2] Calif Dept Hlth Serv, Microbial Dis Lab, Berkeley, CA 94704 USA
[3] Oklahoma Dept Hlth, Oklahoma City, OK 73117 USA
关键词
D O I
10.1128/JCM.39.9.3186-3192.2001
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Francisella tularensis, the etiological agent of tularemia, is found throughout the Northern hemisphere. After analyzing the F. tularensis, genomic sequence for potential variable-number tandem repeats (VNTRs), we developed a multilocus VNTR analysis (MLVA) typing system for this pathogen. Variation was detected at six VNTR loci in a set of 56 Isolates from California, Oklahoma, Arizona, and Oregon and the F. tularensis live vaccine strain. PCR assays revealed diversity at these loci with total allele numbers ranging from 2 to 20, and Nei's diversity index values ranging from 0.36 to 0.93. Cluster analysis identified two genetically distinct groups consistent with the current biovar classification system of F. tularensis. These findings suggest that these VNTR markers are useful for identifying F. tularensis isolates at this taxonomic level. In this study, biovar B isolates were less diverse than those in biovar A, possibly reflecting the history of tularemia in North America. Seven isolates from a recent epizootic in Maricopa County, Ariz., were identical at all VNTR marker loci. Their identity, even at a hypervariable VNTR locus, indicates a common source of infection. This demonstrates the applicability of MLVA for rapid characterization and identification of outbreak isolates. Future construction of reference databases will allow faster outbreak tracking as well as providing a foundation for deciphering global genetic relationships.
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页码:3186 / 3192
页数:7
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