Effects of heavy metals on Pseudomonas aeruginosa and Bacillus thuringiensis

被引:118
作者
Hassen, A
Saidi, N
Cherif, M
Boudabous, A
机构
[1] URNE Eau, Inst Natl Rech Sci & Tech, Tunis 1082, Tunisia
[2] Inst Natl Agron Tunisie, Tunis 1082, Tunisia
[3] Fac Sci Tunis, Microbiol Lab, Tunis 1060, Tunisia
关键词
heavy metals; biosorption; cell mass; proteins; pigments; Pseudomonas aeruginosa; Bacillus thuringiensis;
D O I
10.1016/S0960-8524(98)00011-X
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
The biosorption of the heavy metals most frequently, found in polluted environments by Pseudomonas aeruginosa and Bacillus thuringiensis was studied The effects of these metals on bacterial growth, quantity of dry cells, ammonium assimilation pigment production, and protein synthesis were investigated. At lower concentrations than the minimal inhibitory concentration (MIC), the metals partially limited bacterial growth and caused an inhibition proportional to the metal concentration applied. The production of bacterial biomass varied according to the nature and concentration of the metals, and to the bacterial strain studied The biosorption of metals by P. aeruginosa and B. thuringiensis was variable. Mercury and copper appeared to be the elements most adsorbed by bacteria. Citrate noticeably increased the biosorption of chromium by P. aeruginosa (0.07-45.9%) and copper by B. thuringiensis (18.7-33.8%). Metallic cations everted variable effects on protein synthesis. Zinc stimulated protein synthesis in P. aeruginosa, and cadmium inhibited it significantly in B. thuringiensis. Mercury and cobalt, at concentrations below the MIG, always inhibited the synthesis of pigments in P. aeruginosa. The strong interactions of mercury and copper with organic matter suggest that these undesirable elements might be removed from the environment by bacterial trapping and sequestration. A better understanding of the different for-ms of metals actually existing in polluted environments (speciation) would be of gr eat interest. (C) 1998 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:73 / 82
页数:10
相关论文
共 27 条
[1]   ASSESSMENT OF HEAVY-METAL EQUILIBRIA IN SEWAGE SLUDGE-TREATED SOIL [J].
BEHEL, D ;
NELSON, DW ;
SOMMERS, LE .
JOURNAL OF ENVIRONMENTAL QUALITY, 1983, 12 (02) :181-186
[2]   DEPOSIT OF ZINC AND MANGANESE IN AN AQUEOUS ENVIRONMENT MEDIATED BY MICROBIAL MATS [J].
BENDER, J ;
WASHINGTON, JR ;
GRAVES, B ;
PHILLIPS, P ;
ABOTSI, G .
WATER AIR AND SOIL POLLUTION, 1994, 75 (3-4) :195-204
[3]   EFFECTS OF CADMIUM, COPPER, MAGNESIUM, AND ZINC ON THE DECOMPOSITION OF CITRATE BY A KLEBSIELLA SP [J].
BRYNHILDSEN, L ;
ROSSWALL, T .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1989, 55 (06) :1375-1379
[4]  
CAPELLE R, 1960, CHIM ANALYT, V42, P127
[5]  
CAPELLE R, 1960, CHIM ANALYT, V42, P69
[6]   INTERACTION OF MERCURIC IONS WITH THE BACTERIAL-GROWTH MEDIUM AND ITS EFFECTS ON ENZYMATIC REDUCTION OF MERCURY [J].
CHANG, JS ;
HONG, J ;
OGUNSEITAN, OA ;
OLSON, BH .
BIOTECHNOLOGY PROGRESS, 1993, 9 (05) :526-532
[7]  
DERORE H, 1994, FEMS MICROBIOL ECOL, V14, P263, DOI 10.1111/j.1574-6941.1994.tb00112.x
[8]  
DOELMAN P, 1994, BIOL FERT SOILS, V17, P177, DOI 10.1007/BF00336319
[9]  
GOBLENZ A, 1994, FEMS MICROBIOL REV, V14, P303
[10]  
GUZZO A, 1994, FEMS MICROBIOL REV, V14, P369