Regulation of superoxide dismutase synthesis in Candida albicans

被引：8

作者：

Gunasekaran, U ^{[1
]}

Yang, RP ^{[1
]}

Gunasekaran, M ^{[1
]}

机构：

[1] Fisk Univ, Dept Biol, Nashville, TN 37208 USA

来源：

MYCOPATHOLOGIA | 1998年 / 141卷 / 02期

基金：

美国国家科学基金会;

关键词：

Candida albicans; superoxide dismutase; (SOD); yeast;

D O I：

10.1023/A:1006985710874

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The synthesis of superoxide dismutase [SOD: EC 1.15.1.1] in response to various cultural conditions was examined in Candida albicans, an opportunistic yeast which causes candidiasis in immunosuppressed patients. SOD plays an important role in protecting cells from the oxidative damage of superoxide radicals. Maximum SOD activity was found after 72 hrs of yeast growth. The optimum pH and temperature for the SOD activity were 7 and 40 degrees C, respectively. The major SOD activity was found in the cytosol fraction and the level of extracellular SOD was very low. The enzyme was stimulated to varying degrees by cholic acid, procaine and tocopherol. On the basis of inhibitor studies and other enzyme properties, the isolated enzyme from C. albicans is identified as copper and zinc superoxide dismutase.

引用

页码：59 / 63

页数：5

共 19 条

[1] A STUDY OF THE PH-DEPENDENCE OF THE ACTIVITY OF PORCINE CU,ZN SUPEROXIDE-DISMUTASE [J].