Important role of glutathione in protecting against menadione-induced cytotoxicity in rat platelets

被引：3

作者：

Cho, YS ^{[1
]}

Seung, SA ^{[1
]}

Kim, MJ ^{[1
]}

Lee, JY ^{[1
]}

Chung, JH ^{[1
]}

机构：

[1] SEOUL NATL UNIV,COLL PHARM,SEOUL 151742,SOUTH KOREA

来源：

ARCHIVES OF PHARMACAL RESEARCH | 1996年 / 19卷 / 01期

关键词：

menadione; platelets; glutathione; cytotoxicity;

D O I：

10.1007/BF02976813

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Our previous studies demonstrate that menadione (MEN) is cytotoxic to platelets of rats by depleting glutathione (GSH). In order to clarify whether GSH has a role in protecting against menadione-induced cytotoxicity, the effect of GSH depletors as well as GSH precursors on menadione-induced cytotoxicity was investigated. Cysteine and dithiothreitol (DTT) prevent MEN-induced cytotoxicity in a dose-dependent manner, as determined by LDH leakage and change in turbidity. When platelets were treated with 1-chloro-2,4-dinitrobenzene (CDNB) and diethylmaleate (DEM), both of which deplete intracellular GSH, MEN-induced cy totoxicity was potentiated in the CDNB-treated platelets, but not in the DEM-treated platelets. These data suggest that the GSH in platelets plays an important role in protecting against cytotoxicity induced by menadione.

引用

页码：12 / 17

页数：6

共 18 条

[1] INVOLVEMENT OF THE CYSTATHIONINE PATHWAY IN THE BIOSYNTHESIS OF GLUTATHIONE BY ISOLATED RAT HEPATOCYTES [J].