Effect of oscillatory high hydrostatic pressure treatments on Byssochlamys nivea ascospores suspended in fruit juice concentrates

The effect of continuous (689 MPa with holding times of 5, 15 or 25 min) and oscillatory (one, three or five cycles at 689 MPa with holding times of 1 s) high hydrostatic pressure treatments on the viability of Byssochlamys nivea ascospores suspended in apple and cranberry juice concentrates adjust-ed by dilution to water activities (a(w)) of 0.98 and 0.94 was evaluated at 21 and 60 degrees C, Inactivation of the initial spore inocula was achieved after three or five cycles of oscillatory pressurization at 60 degrees C when the a(w) was 0.98 in both fruit juices. With a(w) 0.94, the initial inocula were reduced by less than 1 log-cycle after five pressure cycles, Inactivation was not observed within 25 min with continuous pressurization at 60 degrees C, In treatments at 21 degrees C, no effect on spore viability was observed with continuous or oscillatory treatments.