Affinity capture of uropathogenic Escherichia coli using pigeon ovalbumin-bound Fe3O4@Al2O3 magnetic nanoparticles

被引:108
作者
Liu, Jr-Chi [1 ]
Tsai, Pei-Jane
Lee, Yuan C. [2 ]
Chen, Yu-Chie [1 ]
机构
[1] Natl Chiao Tung Univ, Dept Appl Chem, Hsinchu 300, Taiwan
[2] Johns Hopkins Univ, Dept Biol, Baltimore, MD 21218 USA
关键词
D O I
10.1021/ac800487v
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Escherichia coli and Staphylococcus saprophyticus are the most common causes of urinary tract infections, with 80% of these infections caused by uropathogenic E. coli. Because the P fimbriae of E. coli have specificity toward Gal(alpha 1-4)Gal beta units, pigeon ovalbumin (POA), whose structure contains terminal Gal(alpha 1-4)Gal beta moieties, was used as a probe for interaction with P fimbriated E. coli. The functional affinity probes for these bacteria by immobilizing POA-a phosphoprotein-onto the surface of magnetic iron oxide nanoparticles (NPs) coated with alumina (Fe3O4@Al2O3), using the phosphate units of POA as linking groups for the formation of phosphate-alumina complexes. The immobilization process occurred within 30 s when performing the reaction under microwave heating. The magnetic POA-Fe3O4@Al2O3 NPs generated using this facile approach exhibited specificity toward P fimbriated E. coli. The bacteria targeted by the affinity probes were characterized by matrix-assisted laser desorption/ionization mass spectrometry. The detection limit toward uropathogenic bacteria when using this approach was similar to 9.60 x 10(4) cfu/mL (0.5 mL).
引用
收藏
页码:5425 / 5432
页数:8
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