Production of deuterated zeaxanthin by Flavobacterium multivorum and its detection by resonance Raman and mass spectrometric methods

被引：7

作者：

Bhosale, P

Teredesai, PV

Jin, LH

Ermakov, IV

Gellermann, W

Bernstein, PS

机构：

[1] Univ Utah, Sch Med, Moran Eye Ctr, Dept Ophthalmol & Visual Sci, Salt Lake City, UT 84132 USA

[2] Univ Utah, Dept Phys, Salt Lake City, UT 84112 USA

来源：

BIOTECHNOLOGY LETTERS | 2005年 / 27卷 / 21期

关键词：

deuterated zeaxanthin; flavobacterium multivorum; high pressure liquid chromatography/atmospheric pressure chemical ionization-mass spectrometry; resonance Raman spectroscopy; zeaxanthin;

D O I：

10.1007/s10529-005-2737-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Flavobacterium multivorum, a zeaxanthin-producing organism, was grown aerobically in a medium prepared with deuterated water. Atmospheric pressure chemical ionization mass spectrometry (APCI-MS) and resonance Raman spectroscopy (RRS) analysis revealed similar to 75% replacement of hydrogen by deuterium atoms as indicated by the molecular mass cluster at around m/z 600. Deuterated zeaxanthin upon excitation with a 488 nm laser exhibited characteristic resonance Raman vibrational modes at 1161 and 1504 cm(-1) as compared to 1007, 1159 and 1525 cm(-1) for undeuterated zeaxanthin. HPLC/APCI-MS and HPLC/RRS were specific and sensitive with limits of detection of 2.5 pg and 50 ng, respectively.

引用

页码：1719 / 1723

页数：5

共 6 条

[1] Identification and quantitation of carotenoids and their metabolites in the tissues of the human eye [J].