Doping of carbon nanotubes with nitrogen improves protein coverage whilst retaining correct conformation

被引：14

作者：

Burch, Hilary J. ^{[1
]}

Contera, Sonia Antoranz ^{[1
]}

de Planque, Maurits R. R. ^{[1
]}

Grobert, Nicole ^{[2
]}

Ryan, J. F. ^{[1
]}

机构：

[1] Univ Oxford, Dept Phys, Clarendon Lab, Bionanotechnol IRC, Oxford OX1 3PU, England

[2] Univ Oxford, Dept Mat, Oxford OX1 3PH, England

来源：

NANOTECHNOLOGY | 2008年 / 19卷 / 38期

关键词：

D O I：

10.1088/0957-4484/19/38/384001

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Relevant parameters for non-covalent protein functionalization of carbon nanotubes are explored. Multiwalled carbon nanotubes are carboxylated and functionalized with metalloproteins. Using atomic force microscopy (AFM) we quantitatively determine that coverage with nitrogen-doped multiwalled carbon nanotubes is superior compared to coverage with un-doped multiwalled carbon nanotubes, due to enhanced carboxylation. Conformational analysis using a combination of AFM, antibody binding assays, circular dichroism and UV-visible spectroscopy demonstrates that the metalloproteins retain their native structure when adsorbed to nitrogen-doped multiwalled carbon nanotubes irrespective of their size, charge or folding motif.

引用

页数：7

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