Molecular cloning, functional characterization and mRNA expression analysis of the murine chemokine receptor CCR6 and its specific ligand MIP-3α

被引：77

作者：

Varona, R

Zaballos, A

Gutiérrez, J

Martín, P

Roncal, F

Albar, JP

Ardavín, C

Márquez, G ^{[1
]}

机构：

[1] Univ Autonoma Madrid, Ctr Nacl Biotecnol, Dept Inmunol & Oncol, E-28049 Madrid, Spain

[2] Univ Complutense Madrid, Fac Biol, Dept Biol Celular, E-28040 Madrid, Spain

来源：

FEBS LETTERS | 1998年 / 440卷 / 1-2期

关键词：

receptor cloning; chemokine cloning; dendritic cell;

D O I：

10.1016/S0014-5793(98)01450-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cloned the murine CCR6 receptor and its ligand, the beta-chemokine mMIP-3 alpha. Calcium mobilization assays performed with mCCR6 transfectants showed significant responses upon addition of mMIP-3 alpha. Murine MIP-3 alpha RNA is expressed in thymus, small intestine and colon, whereas mCCR6 RNA is expressed in spleen and lymph nodes. RT-PCR analysis of FACS-sorted lymphoid and antigen presenting cell subsets showed mCCR6 expression mainly in B cells, CD8(-) splenic dendritic cells and CD4(+) T cells, The cloning and functional characterization of the mCCR6 and mMIP-3 alpha will allow the study of the role of these proteins in mouse models of inflammation and immunity. (C) 1998 Federation of European Biochemical Societies.

引用

页码：188 / 194

页数：7

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