Porous composite scaffold incorporating osteogenic phytomolecule icariin for promoting skeletal regeneration in challenging osteonecrotic bone in rabbits

被引：237

作者：

Lai, Yuxiao ^{[1
]}

Cao, Huijuan ^{[1
,2
]}

Wang, Xinluan ^{[1
,3
,4
]}

Chen, Shukui ^{[1
]}

Zhang, Ming ^{[1
]}

Wang, Nan ^{[1
]}

Yao, Zhihong ^{[5
]}

Dai, Yi ^{[5
]}

Xie, Xinhui ^{[3
,4
,6
]}

Zhang, Peng ^{[1
,2
]}

Yao, Xinsheng ^{[5
]}

Qin, Ling ^{[1
,3
,4
]}

机构：

[1] Chinese Acad Sci, Shenzhen Inst Adv Technol, Inst Biomed & Hlth Engn, Translat Med R&D Ctr, Shenzhen 518055, Peoples R China

[2] Shenzhen Bioact Mat Engn Lab Med, Shenzhen 518055, Peoples R China

[3] Chinese Univ Hong Kong, Musculoskeletal Res Lab, Dept Orthopaed & Traumatol, Hong Kong, Hong Kong, Peoples R China

[4] Chinese Univ Hong Kong, Innovat Orthopaed Biomat & Drug Translat Res Lab, Li Ka Shing Inst Hlth, Hong Kong, Hong Kong, Peoples R China

[5] Jinan Univ, Coll Pharm, Inst Tradit Chinese Med & Nat Prod, Guangzhou 510632, Guangdong, Peoples R China

[6] Southeast Univ, Sch Med, ZhongDa Hosp, Dept Orthoped, Nanjing, Jiangsu, Peoples R China

来源：

BIOMATERIALS | 2018年 / 153卷

基金：

中国国家自然科学基金;

关键词：

Steroid-associated osteonecrosis(SAON); Porous scaffold; Osteogenesis; 3D printing; Bone regeneration; STEROID-ASSOCIATED OSTEONECROSIS; FEMORAL-HEAD; IN-VITRO; TISSUE; DIFFERENTIATION; RELEASE; REPAIR; BIOMATERIALS; ANGIOGENESIS; COMBINATION;

D O I：

10.1016/j.biomaterials.2017.10.025

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

Steroid-associated osteonecrosis (SAON) often requires surgical core decompression (CD) in the early stage for removal of necrotic bone to facilitate repair where bone grafts are needed for filling bone defect and avoiding subsequent joint collapse. In this study, we developed a bioactive composite scaffold incorporated with icariin, a unique phytomolecule that can provide structural and mechanical support and facilitate bone regeneration to fill into bone defects after surgical CD in established SAON rabbit model. An innovative low-temperature 3D printing technology was used to fabricate the poly (lactic-co-glycolic acid)/beta-calcium phosphate/icariin (PLGA/TCP/Icariin, PTI) scaffold. The cytocompatibility of the PTI scaffold was tested in vitro, and the osteogenesis properties of PTI scaffolds were assessed in vivo in the SAON rabbit models. Our results showed that the fabricated PTI scaffold had a well-designed biomimic structure that was precisely printed to provide increased mechanical support and stable icariin release from the scaffold for bone regeneration. Furthermore, our in vivo study indicated that the PTI scaffold could enhanced the mechanical properties of new bone tissues and improved angiogenesis within the implanted region in SAON rabbit model than those of PLGA/TCP (PT) scaffold. The underlying osteoblastic mechanism was investigated using MC3T3-E1 cells in vitro and revealed that icariin could facilitate MC3T3-E1 cells ingrowth into the PTI scaffold and regulate osteoblastic differentiation. The PTI scaffold exhibited superior biodegradability, biocompatibility, and osteogenic capability compared with those of PT scaffold. In summary, the PTI composite scaffold which incorporated bioactive phytocompounds is a promising potential strategy for bone tissue engineering and regeneration in patients with challenging SAON. (C) 2017 Elsevier Ltd. All rights reserved.

引用

页码：1 / 13

页数：13

共 57 条

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