PLK1 protects against sepsis-induced intestinal barrier dysfunction

被引：30

作者：

Cao, Yingya ^{[1
]}

Chen, Qun ^{[1
]}

Wang, Zhen ^{[1
]}

Yu, Tao ^{[1
]}

Wu, Jingyi ^{[1
]}

Jiang, Xiaogan ^{[1
]}

Jin, Xiaoju ^{[1
]}

Lu, Weihua ^{[1
]}

机构：

[1] Yijishan Hosp, Wannan Med Coll, Dept Intens Care Unit, Wuhu 241001, Anhui, Peoples R China

来源：

SCIENTIFIC REPORTS | 2018年 / 8卷

关键词：

KINASE; 1; APOPTOSIS; PERMEABILITY; SURVIVAL; INHIBITION; TARGET;

D O I：

10.1038/s41598-018-19573-x

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Sepsis and sepsis-associated intestinal barrier dysfunction are common in intensive care units, with high mortality. The aim of this study is to investigate whether Polo-like kinase 1 (PLK1) ameliorates sepsis-induced intestinal barrier dysfunction in the intestinal epithelium. The mouse intestinal barrier was disrupted after Lipopolysaccharide (LPS) injection due to intestinal epithelial cell apoptosis and proliferation inhibition, accompanied by decreased PLK1. In HT-29 intestinal epithelial cells, LPS stimulation induced cell apoptosis and inhibited cell proliferation. Overexpression of PLK1 partly rescued the apoptosis and proliferation inhibition in HT29 cells caused by LPS. Finally, LPS stimulation promoted the reduction of PLK1, resulting in apoptosis and proliferation inhibition in intestinal epithelial cells, disrupting the intestinal epithelial barrier. These findings indicate that PLK1 might be a potential therapeutic target for the treatment of sepsis-induced intestinal barrier dysfunction.

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页数：8

相关论文

共 30 条

[1]

TNF-α/Cycloheximide-Induced Oxidative Stress and Apoptosis in Murine Intestinal Epithelial MODE-K Cells [J].

Babu, Dinesh ;

Soenen, Stefaan J. ;

Raemdonck, Koen ;

Leclercq, Georges ;

De Backer, Ole ;

Motterlini, Roberto ;

Lefebvre, Romain A. .

CURRENT PHARMACEUTICAL DESIGN, 2012, 18 (28) :4414-4425

[2]

Oncostatin M Mediates STAT3-Dependent Intestinal Epithelial Restitution via Increased Cell Proliferation, Decreased Apoptosis and Upregulation of SERPIN Family Members [J].

Beigel, Florian ;

Friedrich, Matthias ;

Probst, Corina ;

Sotlar, Karl ;

Goeke, Burkhard ;

Diegelmann, Julia ;

Brand, Stephan .

PLOS ONE, 2014, 9 (04)

[3]

LPS Induces Hyper-Permeability of Intestinal Epithelial Cells [J].

Bein, Amir ;

Zilbershtein, Alexander ;

Golosovsky, Michael ;

Davidov, Dan ;

Schwartz, Betty .

JOURNAL OF CELLULAR PHYSIOLOGY, 2017, 232 (02) :381-390

[4]

Intestinal permeability - a new target for disease prevention and therapy [J].

Bischoff, Stephan C. ;

Barbara, Giovanni ;

Buurman, Wim ;

Ockhuizen, Theo ;

Schulzke, Joerg-Dieter ;

Serino, Matteo ;

Tilg, Herbert ;

Watson, Alastair ;

Wells, Jerry M. .

BMC GASTROENTEROLOGY, 2014, 14

[5]

Amelioration of IFN-γ and TNF-α-Induced Intestinal Epithelial Barrier Dysfunction by Berberine via Suppression of MLCK-MLC Phosphorylation Signaling Pathway [J].

Cao, Min ;

Wang, Pei ;

Sun, Chunhong ;

He, Wen ;

Wang, Fengjun .

PLOS ONE, 2013, 8 (05)

[6]

Small molecule inhibition of polo-like kinase 1 by volasertib (BI 6727) causes significant melanoma growth delay and regression in vivo [J].

Cholewa, Brian D. ;

Ndiaye, Mary A. ;

Huang, Wei ;

Liu, Xiaoqi ;

Ahmad, Nihal .

CANCER LETTERS, 2017, 385 :179-187

[7]

Intestinal epithelium in inflammatory bowel disease [J].

Coskun, Mehmet .

FRONTIERS IN MEDICINE, 2014, 1

[8]

From Plk1 to Plk5 Functional evolution of Polo-like kinases [J].

de Carcer, Guillermo ;

Manning, Gerard ;

Malumbres, Marcos .

CELL CYCLE, 2011, 10 (14) :2255-2262

[9]

Overexpression of PLK1 is associated with poor survival by inhibiting apoptosis via enhancement of survivin level in esophageal squamous cell carcinoma [J].

Feng, Yan-Bin ;

Lin, De-Chen ;

Shi, Zhi-Zhou ;

Wang, Xiao-Chun ;

Shen, Xiao-Ming ;

Zhang, Yu ;

Du, Xiao-Li ;

Luo, Man-Li ;

Xu, Xin ;

Han, Ya-Ling ;

Cai, Yan ;

Zhang, Zi-Qiang ;

Zhan, Qi-Min ;

Wang, Ming-Rong .

INTERNATIONAL JOURNAL OF CANCER, 2009, 124 (03) :578-588

[10]

Epithelial-Mesenchymal Transition Predicts Polo-Like Kinase 1 Inhibitor-Mediated Apoptosis in Non-Small Cell Lung Cancer [J].

Ferrarotto, Renata ;

Goonatilake, Ruchitha ;

Yoo, Suk Young ;

Tong, Pan ;

Giri, Uma ;

Peng, Shaohua ;

Minna, John ;

Girard, Luc ;

Wang, Yuehong ;

Wang, Liguang ;

Li, Lerong ;

Diao, Lixia ;

Peng, David H. ;

Gibbons, Don L. ;

Glisson, Bonnie S. ;

Heymach, John V. ;

Wang, Jing ;

Byers, Lauren A. ;

Johnson, Faye M. .

CLINICAL CANCER RESEARCH, 2016, 22 (07) :1674-1686