A simple method of DNA extraction from soil for detection of composite transgenic plants by PCR

被引：11

作者：

Ernst, D

Kiefer, E

Drouet, A

Sandermann, H

机构：

[1] GSF,INST BIOCHEM PFLANZENPATHOL,D-85758 OBERSCHLEISSHEIM,GERMANY

[2] UNIV ORLEANS,LAB BIOL LIGNEUX FORESTIERS,F-45067 ORLEANS 2,FRANCE

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 1996年 / 14卷 / 02期

关键词：

DNA extraction; PCR; soil; transgene;

D O I：

10.1007/BF02684903

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This new and simple method of DNA extraction from composite soil allows the isolation of plant DNA with high efficiency, quality and reproductivity. The method is based on a simple CaCl2-precipitation step and requires no additional purification steps to eliminate humic acids. The extracted DNA was obtained in sufficient purity and quantity to allow direct detection of transgenes by PCR. Furthermore, the simple procedure allows the assay of many samples at the same time.

引用

页码：143 / 148

页数：6

共 14 条

[1]

BECKER J, 1994, BIOL SICHERHEIT, V3, P563

[2]

CASPER R, 1992, BIOSAFETY RESULTS FI

[3]

DONN G, 1992, BIOENGINEERING, V5, P40

[4]

DONN G, 1992, BIOENGINEERING, V6, P40