Microbiome complexity and Staphylococcus aureus in chronic rhinosinusitis

被引：213

作者：

Feazel, Leah M. ^{[1
]}

Robertson, Charles E. ^{[4
]}

Ramakrishnan, Vijay R. ^{[2
]}

Frank, Daniel N. ^{[1
,3
]}

机构：

[1] Univ Colorado, Sch Med, Div Infect Dis, Aurora, CO USA

[2] Univ Colorado, Sch Med, Dept Otolaryngol Head & Neck Surg, Aurora, CO USA

[3] Univ Colorado, Sch Med, Microbiome Res Consortium, Aurora, CO USA

[4] Univ Colorado, Dept Mol Cellular & Dev Biol, Boulder, CO 80309 USA

来源：

LARYNGOSCOPE | 2012年 / 122卷 / 02期

关键词：

Bacteria; bacterial sinusitis; human microbiome; sinus culture; middle meatus; Level of Evidence: 2b; RAPID DETECTION; BACTERIAL; SINUSITIS; INFECTION; BIOFILMS; PRIMERS; ASSAY;

D O I：

10.1002/lary.22398

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

100103 [病原生物学]; 100218 [急诊医学];

摘要：

Objectives/Hypothesis: The aim of this study was to compare microbiological culture-based and culture-independent (16S rRNA gene sequencing) methodologies for pathogen identification in chronic rhinosinusitis (CRS) patients. We hypothesized that bacterial culture and DNA sequencing would yield largely concurrent results, although sequencing would detect greater bacterial diversity, and the sinonasal microbiomes of CRS patients would differ in composition and diversity compared with non-CRS controls.

引用

页码：467 / 472

页数：6

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