Using 2′, 7′-dichlorodihydrofluorescein-diacetate to assess polysaccharides as immunomodulating agents

被引：18

作者：

Bland, EJ ^{[1
]}

Keshavarz, T ^{[1
]}

Bucke, C ^{[1
]}

机构：

[1] Univ Westminster, Sch Biosci, Fungal Biotechnol Grp, London W1W 6UW, England

来源：

MOLECULAR BIOTECHNOLOGY | 2001年 / 19卷 / 02期

关键词：

2; 7; '-dichlorodihydrofluorescein-diacetate; reactive oxygen species; polysaccharides; polymorphonuclear leukocytes; neutrophils; immunomodulation;

D O I：

10.1385/MB:19:2:125

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Neutrophils play a key role in the defense against microbial infections. One of their primary weapons to destroy microbes is the production of reactive oxygen species (ROS). This paper shows how 2', 7'-dichlorodihydrofluorescein-diacetate (DCFH-DA) was used to measure the effects of polysaccharides on the production of ROS by polymorphonuclear leukocytes (PMNs). The DCFH-DA method has been designed to provide a highly sensitive, quantifiable, real-time assessment of ROS production by PMNs.

引用

页码：125 / 131

页数：7

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