Optimisation of mobile phase pH in liquid chromatography.: Application to fluorimetric detection of series of quinolones

被引：18

作者：

Hernández-Arteseros, JA ^{[1
]}

Barbosa, J ^{[1
]}

Compañó, R ^{[1
]}

Prat, MD ^{[1
]}

机构：

[1] Univ Barcelona, Dept Quim Analit, E-08028 Barcelona, Spain

来源：

CHROMATOGRAPHIA | 1998年 / 48卷 / 3-4期

关键词：

column liquid chromatography; mobile phase optimisation; fluorimetric detection; quinolones; dissociation constants;

D O I：

10.1007/BF02467679

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The dissociation constants of several quinolones in a 7 % acetonitrile-water mixture are determined. A model describing the effect of pH on the retention of quinolones by an octadecylsilica stationary phase with a 7 % acetonitrile-water mobile phase is proposed. The model uses pH values in the mobile phase instead of pH values in water and takes into account the effect of activity coefficients. Fluorescence of quinolones in the mobile phase is studied, and a chromatographic separation with fluorimetric detection is established. Detection limits for the proposed method range from 3 to 12 mu g.L-1.

引用

页码：251 / 257

页数：7

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