Inheritance characteristics of microsatellite DNA loci in experimental families of Japanese flounder Paralichthys olivaceus

被引：14

作者：

Sekino, M

Hara, M

机构：

[1] Tohoku Natl Fisheries Res Inst, Shiogama, Miyagi 9850001, Japan

[2] Natl Res Inst Aquaculture, Nansei, Mie 5160193, Japan

来源：

MARINE BIOTECHNOLOGY | 2001年 / 3卷 / 04期

关键词：

microsatellite DNA loci; Japanese flounder; Paralichthys olivaceus; Mendelian inheritance;

D O I：

10.1007/s10126-001-0052-z

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Allele segregating patterns of microsatellite DNA loci in 5 experimental families of Japanese flounder Paralichthys olivaceus and genotype frequencies for deviation from Hardy-Weinberg expectations (HWE) in natural P. olivaceus populations were studied to assess inheritability. Of the 12 microsatellite loci examined, I locus had a possibility of scoring errors of heterozygous individuals caused by unreproducible polymerase chain reaction amplifications of a particular allele. At the remaining 11 loci, almost all of alleles were segregated according to Mendelian transmission, and observed genotype frequencies in natural populations were consistent with HWE. The results demonstrated here would provide useful information supporting the suitability of these microsatellite loci as inheritable P. olivaceus genetic markers.

引用

页码：310 / 315

页数：6

共 12 条

[1] Tissue preservation and total DNA extraction from fish stored at ambient temperature using buffers containing high concentration of urea [J].