Optimized liquid chromatography-mass spectrometry approach for the isolation of minor stress biomarkers in plant extracts and their identification by capillary nuclear magnetic resonance

被引：80

作者：

Glauser, Gaetan ^{[1
,2
]}

Guillarme, Davy ^{[2
,3
]}

Grata, Elia ^{[1
,2
,3
]}

Boccard, Julien ^{[2
,3
,4
]}

Thiocone, Aly ^{[1
,2
]}

Carrupt, Pierre-Alain ^{[2
,4
]}

Veuthey, Jean-Luc ^{[2
,3
]}

Rudaz, Serge ^{[2
,3
]}

Wolfender, Jean-Luc ^{[1
,2
]}

机构：

[1] Univ Geneva, EPGL, Sch Pharmaceut Sci, Lab Pharmacognosy & Phytochem, CH-1211 Geneva 4, Switzerland

[2] Univ Lausanne, CH-1211 Geneva, Switzerland

[3] Univ Geneva, EPGL, Sch Pharmaceut Sci, Lab Pharmaceut Analyt Chem, CH-1211 Geneva 4, Switzerland

[4] Univ Geneva, EPGL, Sch Pharmaceut Sci, LCT Pharmacochem, CH-1211 Geneva 4, Switzerland

来源：

JOURNAL OF CHROMATOGRAPHY A | 2008年 / 1180卷 / 1-2期

关键词：

MS-directed semi-preparative LC; UPLC-TOF-MS; geometrical method transfer; CapNMR; metabolomics; Arabidopsis thaliana; hydroxylated jasmonates; wound biomarkers;

D O I：

10.1016/j.chroma.2007.12.021

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A LC-MS approach is presented for the isolation of minor key plant biomarkers, in view of their characterization by NMR at the microgram scale. Due to the complexity of plant extracts, the purification of metabolites present in low concentrations is critical. The strategy used relies on the optimization of the chromatographic analysis using ultra-performance liquid chromatography-time-of-flight mass spectrometry (UPLC-TOF-MS), thanks to modelling software. The optimized method is then transferred to semi-preparative LC conditions with MS detection. The approach is illustrated by the isolation of wound-induced jasmonate derivatives revealed by a metabolomic study in Arabidopsis thaliana leaves and their subsequent characterization by capillary NMR (CapNMR). (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：90 / 98

页数：9

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